The Escherichia coli detection kit is based on the fast, sensitive, and proven primers/probe qPCR technique. The kit contains primers and a probe for detecting a highly specific sequence (uidA gene) present on the DNA of the species Escherichia coli. This kit of Sylphium molecular ecology is designed to have a sensitivity of only one molecule per reaction in environmental samples.
Toxin information
Escherichia coli, also known as E. coli , is a Gram-negative, facultative anaerobic, rod-shaped, coliform bacterium of the genus Escherichia that is commonly found in the lower intestine of warm-blooded organisms (endotherms).Most E. coli strains are harmless, but some serotypes can cause serious food poisoning in their hosts, and are occasionally responsible for product recalls due to food contamination.
Primer design
Primers and probe and the qPCR buffer were designed to have:
The kit is developed and optimized to be used on eDNA isolates purified using the eDNA isolation kit (#SYL002) from Sylphium molecular ecology.
Kit contents
The kit contains materials for an 3-fold analyses on 36 samples including all necessary controls.
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