The Pseudorasbora parva (stone moroko) eDNA detection kit is able to detect the fish species Pseudorasbora parva in all waters worldwide and contains enough materials for 20 samples to be analysed in 8 fold (200 qPCR tests). The kit is based on the fast, sensitive, and proven primers/probe qPCR technique. The kit contains primers and a probe for detecting a highly specific sequence present on the mitochondrial DNA of the species Pseudorasbora parva.
Species information
The stone moroko (Pseudorasbora parva), also known as the topmouth gudgeon, is a fish belonging to the Cyprinid family, native to Asia, but introduced and now considered an invasive species in Europe. The fish’s size is rarely above 8 cm and usually 2 to 7.5 cm long.
The fish was introduced in the 1960s into ponds in Nucet, Dâmboviţa County, Romania and it made its way into Danube, then spreading throughout Europe. They pose danger to other species such as the sunbleaks (Leucaspius delineatus). They are the carrier of a parasite (Sphaerothecum destruens) that is not damaging to the topmouth gudgeon, but attacks other fishes like the sunbleaks, which are unable to spawn and have a higher mortality when infected.[4] They also feed on eggs of locally valuable native fish species.
The species has also been recently discovered in several lakes in the UK where it is believed to have been illegally stocked. This has called for a large scale eradication programme organised by the Environment Agency who kill the fish off with a piscicide called rotenone.
In English: stone moroko or topmouth gudgeon.
In German: Blaubandbärbling.
In French:
In Dutch: Blauwband.
rimer design
The primers and probe are specially designed to be used with eDNA samples and have the following properties:
The kit is developed and optimized to be used on eDNA isolates purified using the eDNA isolation kit (#SYL002) from Sylphium molecular ecology.
Kit contents
The kit contains materials for an 8 fold analyses on 20 samples including all necessary controls.
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