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    eDNA Isolatiekit - Met of Zonder Interne Positieve Controle

    €100,43
    • Inclusief 21% btw: €100,43
    • Exclusief btw: €83,-
    0 Op voorraad: Dropshipping: doorgaans binnen 2 werkdagen
    De eDNA Isolatiekit wordt gebruikt om snel en efficiënt eDNA uit watermonsters te isoleren.... Toon meer
    • Gratis verzending in Nederland vanaf €35,-
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    Productomschrijving

    The Environmental DNA Isolation Kit is used to quickly and efficiently isolate environmental DNA from water samples. The Environmental DNA Isolation Kit procedure is suitable for qualitative and quantitative PCR amplification. The whole procedure for isolation of total eDNA takes about 2 hours. The isolated DNA will be dissolved in TE and is ready to use in qPCR.

    The kit is perfectly suited for eDNA extraction and isolation from the eDNA Dual Filter membrane. See a short tutorial how to extract preserved eDNA from the capsule and how to proceed. The complete procedure is described in the instruction protocol.

    Optional:  internal positive control (IPC) quantification kit

     All Sylphium sample sets contain an internal positive control (IPC) in the preservation solution. The internal control consists of a small piece of synthetic xenobiotic DNA. The positive control DNA sequence selected is unknown in the aquatic environment and will not interfere with the detection of target organisms. The internal positive control is an efficiency check of the DNA isolation procedure and a quality and purity check of the isolated DNA. Optionally, the Internal Positive Control (IPC) quantification kit can be ordered along with the eDNA Isolation Kit. The IPC is quantified through the FAM channel.

    Kit contents DNA isolation kit

    • S1: Internal negative control
    • S2: Phase separation solution
    • S3: Precipitation buffer
    • S4: Precipitation solution
    • S5: Wash solution
    • S6: eDNA conservation buffer

    Kit contents Internal positive control qPCR detection kit (Optional)

    • Positive control
    • eTaq qPCR mastermix
    • Primer/probe mix for detection of the internal positive control (FAM dye)
    • eTaq DNA polymerase

    Equipment and consumables required

    • qPCR machine  capable for detection of the FAM dye (optional)
    • Microcentrifuge (13,000 x g)
    • Pipettors
    • -20 °Freezer
    • PCR cooling rack (optional)
    • Centrifuge for 15 ml tubes
    • Incubator or heat block at 55 °C
    • 15 ml tubes with cap
    • 2 ml tubes with lit
    • 96 wells qPCR plates + seal cover (optional)

    Specificaties

    Artikelnummer
    SYL002/20/000

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    eDNA Isolatiekit - Met of Zonder Interne Positieve Controle
    € 100,43
    eDNA Isolatiekit - Met of Zonder Interne Positieve Controle
    Aantal: 20 Isolaten, Wel/geen IPC: Zonder interne positieve controle
    0 Op voorraad: Dropshipping: doorgaans binnen 2 werkdagen
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