The Epidalea calamita (Natterjack toad) eDNA detection kit is able to detect the amphibian species Epidalea calamita in all waters worldwide and contains enough materials for 20 samples to be analysed in 8 fold (200 qPCR tests). The kit is based on the fast, sensitive, and proven primers/probe qPCR technique. The kit contains primers and a probe for detecting a highly specific sequence present on the mitochondrial DNA of the species Epidalea calamita.
Species information
The natterjack toad (Epidalea calamita) is a toad native to sandy and heathland areas of Europe. Adults are 60–70 mm in length and are distinguished from common toads by a yellow line down the middle of the back, and parallel paratoid glands. They have relatively short legs, and this gives them a distinctive gait, contrasting with the hopping movement of many other toad species.
Natterjacks have a very loud and distinctive mating call amplified by the single vocal sac found under the chin of the male, so their name literally means the chattering toad – the jack (or toad) that natters. Natterjacks live for up to 15 years and feed mainly on insects, especially beetles. At night they move around open terrain with sparse vegetation, and in loose sand their tracks can often be seen. They move considerable distances each night, enabling the species to colonize new habitats very quickly.
In English: Natterjack toad.
In German: Kreuzkröte.
In French: Crapaud calamite or Crapaud des joncs.
In Dutch: Rugstreeppad.
Primer design
The primers and probe are specially designed to be used with eDNA samples and have the following properties:
The kit is developed and optimized to be used on eDNA isolates purified using the eDNA isolation kit (#SYL002) from Sylphium molecular ecology.
Kit contents
The kit contains materials for an 8 fold analyses on 20 samples including all necessary controls.
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